肿瘤抗原MAGE-12的表位及二级结构预测

Predication of secondary structures and epitopes of tumor antigen MAGE-12

目的预测肿瘤抗原 MAGE- 12的α-螺旋二级结构、B细胞表位和 MAGE- 12的 HL A- A2限制性 CTL表位。方法用 Garnier- Robson和 Chou- Fasman方案预测肿瘤抗原 MAGE- 12的α-螺旋二级结构 ;用 Kyte- Doolittle亲水方案预测肿瘤抗原 MAGE- 12的 B细胞表位 ;用简单基序 ( simple motifs)和延展基序 ( extended m otifs)对肿瘤抗原 MAGE- 12的 HL A-A2限制性 CTL 表位进行预测。结果 MAGE- 12可能含有较多的 α-螺旋 ;B细胞识别的表位可能在 82～ 93残基 ( QSDEGSS-NEEQE)或附近和 3～ 14残基 ( L EQRSQHCKPEE)或附近 ;发现 1个 HL A- A2限制性 CTL 表位为 FL WGPRAL V( 2 71～2 79)。结论用亲水性方案和基序法预测肿瘤抗原 MAGE- 12的 B细胞表位和 MAGE- 12的 HL A- A2限制性 CTL 表位 ,为实验方法探索 MAGE- 12的表位提供有用线索。

ObjectiveTo predict the secondary structures, B-cell epitopes and the HLA-A2 restricted cytotoxic T lymphocyte (CTL) epitope of tumor antigen MAGE-12. Methods The secondary structures of tumor antigen MAGE-12 were predicted by Garnier-Robson's method and the antigenic epitopes of B cells were analysed by using hydrophilicity plot. HLA-A2 restricted CTL epitopes of MAGE-12 were predicted by the method of simple motif and extended motif. Results MAGE-12 are rich in alpha helix;the epitope recognized by B cells may be at 3-14 or adjacent to 82-93;and one HLA-A2 restricted CTL epitope of tumor antigen MAGE-12 is at 271-279(FLWGPRALV). ConclusionB-cell epitopes and CTL epitope of MAGE-12 were predicted by using hydrophilicity approach and motif plots. The predicted epitopes provided useful hints to probe epitopes in MAGE-12 by experiments.