HIV病毒载量定量测定方法的比较研究

A comparative study on testing methods for HIV viral load

[目的 ] 比较不同检测方法对人类免疫缺陷病毒 (HIV )病毒载量间的一致性 ,供临床应用选择方法时参考。[方法 ] 在上海地区对HIV感染者和艾滋病 (AIDS)病人采集 91份标本 ,每份标本同时用bDNA法和NASBA法测定 ,比较两者检出率、相关性和不一致性。 [结果 ] HIV病毒载量检出率均在 80 % ,且无样本错位 ;两种方法检测的HIV病毒载量Log值呈高度线性相关 ,同时发现 7例病人两种方法测得的病毒载量存在明显差异。 [结论 ] bDNA法和NASBA法检测HIV病毒载量具有高度一致性 ,在实际工作中可任意选用 ,NASBA法在HIVRNA低浓度时敏感性更高 。

Objective] To investigate the coincidence among different testing methods for HIV viral load to provide the selection for clinical use in reference. [Methods] From cases with HIV infections and AIDS in Shanghai, 91 plasma samples were collected and tested for HIV viral load, by both bDNA and NASBA. The detection rate, correlation and difference were compared. [Results] The detection rate for HIV viral load among the observed subjects were 80% on average with no difference in duplication. The Log valus of the viral load showed high linear correlation between the results of these two methods. But there was a significant difference among the results of 7 cases with AIDS. [Conclusion] These two testing methods (bDNA and NASBA) for HIV viral load had an very high coincidence for either one of the methods be selected in practice. In fact, the NASBA was more sensitive at low titler of HIV viral load while the bDNA was able to test for HIV subtypes more extensively.