气腹影响肝脏吲哚青绿排泄的实验研究

Effects of pneumoperitoneum on hepatic indocyanine green clearance in rats

目的腹腔镜手术中气腹对机体生理功能的一些影响是已知的。本研究以吲哚青绿(ICG)药代动力学参数作为肝脏血流指数,观察气腹时的变化情况。方法雄性 Wistar 大鼠18只,随机分成三组:麻醉组、开腹组、气腹组。气腹压力为8mmHg,ICG(1mg/kg)股静脉给药,进行 ICG 15 min排泄试验。结果分别测得各组血清 ICG 含量。开腹组 ICG 水平虽高于麻醉组,但无统计学差异(P>0.05)。而气腹组 ICG 水平显著高于麻醉组及开腹组(P<0.05)。结论气腹使 ICG 排泄降低的结果,证实了腹腔镜手术中气腹压力可减少肝脏血流量。

Objective To determine if indocyanine green(ICG)pharmacokinetic parameters,as indices of hepatic blood flow,change during abdominal insufltation.Methods Eighteen male Wistar rats were randomized into 3 groups to receive anesthesia,laparotomy and pneumoperitoneum,respectively.Pneumoperitoneum was per- formed at 8 mmHg.ICG(1mg/kg)was injected into a fermoral vein.ICG clearance test was conducted in all the 3 groups.Results The ICG level was slightly higher in laparotomy group than in anesthesia group(P>0.05). However,the level was significantly higher in pneumoperitoneum group than in the former 2 groups(P<0.05). Conclusions Pneumoperitoneum can be used to decrease liver blood flow during laparoscopic surgery.