无血清培养体系中CD3AK细胞诱导HL-60细胞凋亡的研究

Study on HL-60 cell apoptosis induced by CD3 McAb-activated killer cells in serum-free culture system

目的 :采用无血清培养体系 ,研究CD3单克隆抗体激活的杀伤 (CD3AK)细胞诱导HL 6 0细胞凋亡情况。方法 :用血清代用品代替人血清培养CD3AK细胞 ,将诱导生成的CD3AK细胞与HL 6 0细胞共同孵育后 ,通过观察细胞形态、琼脂糖凝胶电泳检测DNA条带及流式细胞仪 (FCM)检测分析HL 6 0细胞凋亡情况。结果 :与CD3AK细胞孵育 7h后的HL 6 0细胞出现凋亡细胞形态特征 ,DNA凝胶电泳出现典型梯状电泳带 ,FCM检测凋亡细胞比例为 2 4 5 9%。结论 :无血清培养条件下CD3AK细胞可诱导HL 6

Objective:To investigate whether CD3McAb activated killer(CD3AK)cells could induce apoptosis in HL 60 cells in serum free culture system.Methods:HL 60 cells were coincubated with CD3AK cells expanded in serum free medium,then apoptosis was assessed by means of morphological studies,DNA agarose gel electrophoresis and flow cytomether(FCM) analysis.Results:After 7 h of coincubation with CD3AK cells in serum free medium,HL 60 cells showed morphological features of apoptosis and typical DNA ladder on gel electrophoresis.FCM analysis showed that 24.59% cells were found to undergo apoptosis.Conclusion:CD3AK cells can induce apoptosis in HL 60 cells under serum free culture conditions.