乙肝病毒相关性肾炎的HLA基因频率分布

The distribution of gene frequencies of human leucocyte antigen of hepatitis B virus-associated glomerulonephritis

目的 :探讨乙肝病毒相关性肾炎 (HBV GN)与人类白细胞抗原 (HLA)基因系统的相关性。方法 :应用国际通用的HLA标准微量淋巴细胞毒方法检测HBV GN患者的HLA Ⅰ类抗原 ;利用聚和酶链反应 序列特异性引物技术 (PCR SSP)进行HLA Ⅱ类基因分型 ,并与正常人进行比较。结果 :1 发现HBV GN患者HLA A3、A10抗原频率高于正常对照组 (A3:Pc <0 0 1,RR =16 33;A10 :Pc <0 0 1,RR =10 76 )。 2 HLA DRB1 0 3基因频率在HBV GN患者组较正常对照组明显增高 (DRB1 0 3:Pc <0 0 1,RR =12 90 ) ,而HLA DQB1 0 3基因频率较正常对照组明显降低 (DQB1 0 3:Pc <0 0 0 1,RR =0 12 )。结论 :提示乙肝病毒相关性肾炎与HLA A3、A10、DRB1 0 3正相关 ,与HLA DQB1 0

Objective:To investigate the relationship between patients suffering hepatitis B Virus associated glomerulonephritis(HBV GN) and human leucocyte antigen(HLA) Methods: Patients with HBV GN were tested for HLA classⅠantigen and gene frequencies by using the NIH standard microlyphocytotoxicity assay and typed for HLA Ⅱ genes with polymerase chain reaction sequence specific primer(PCR SSP) and their gene frequencies were compared with those of normal controls Results: HLA A3?A10 antigen and gene frequencies with HBV GN were significantly higher in patients than in controls (A3:Pc<0 01,RR=16 33; A10:Pc<0 01,RR=10 76) DRB1*03 gene frequency was markedly higher in patients than in controls (DRB1*03:Pc<0 01,RR=12 90), DQB1*03,gene frequency was significantly lower in the patients(DQB1*03:Pc<0 001,RR=0 12) Conclusion:The results suggested that HBV GN be positively related to HLA A3?A10?DRB1*03 and was negatively related to DQB1*03