摘要
目的 探讨镁对人脐静脉内皮细胞增殖活力的影响及对心血管系统的保护作用。方法 在改进人脐静脉内皮细胞培养方法的基础上 ,用MTT法检测Mg2 +对传 2~ 3代人脐静脉内皮细胞增殖活力的影响 ,实验分为空白对照组 ;H2 O2 组 ;补镁组 ;H2 O2 +Mg2 +组。结果 补镁各剂量组OD值高于空白对照组 ,H2 O2 +Mg2 +各剂量组OD值高于H2 O2 组 ,均具有显著性差异。结论 提示镁对内皮细胞增殖有明显的促进作用。
Objective To explore the effects of magnesium on the proliferative activity of cultured humal umbilical vein endothelial cells and its protective effects on cardiovascular system.Methods The effect of magnesium on the proliferative activity of cultured human uambilical vein endothelial cells which were 2-3 passage. MTT assay was used for the determination of cell viability and proliferation. Results The value of OD\-\{490\} of cells of Mg\+\{2+\} supplementation group at doses of 0.3mmol/L, 0.6mmol/L, 1.2mmol/L, 2.4mmol/L was much higher than that of normal control group, P<0.05, and that OD\-\{490\} of cells of H\-2O\-2+Mg\+\{2+\} group at all doses was higher than that of H\-2O\-2 group,P<0.05.Conclusion Mg\+\{2+\} supplementation stimulate the proliferation of cultured human umbilical vein endothelial cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2001年第9期807-808,共2页
Chinese Journal of Public Health
基金
博士点基金资助 (No .C990 7)
关键词
镁
人脐静脉内皮细胞
细胞增殖
实验研究
magnesium
human umbilical vein endothelial cells
proliferation