兰索拉唑对离体壁细胞酸分泌的影响

Effect of lansoprazole on acid secretion of isolated rabbit parietal cells

目的 应用兔离体壁细胞为模型 ,研究兰索拉唑体外抑酸效果。方法 应用细胞淘洗与连续密度梯度离心相结合的方法分离兔胃黏膜壁细胞 ,以14 C氨基比林摄取为酸分泌指标 ,观察西咪替丁及兰索拉唑对离体壁细胞组胺诱导的酸分泌的影响。结果 壁细胞纯度达 80 %以上进行实验 ,兰索拉唑能明显抑制离体壁细胞组胺诱导的酸分泌 ,对组胺刺激酸分泌的 5 0 %抑制量 (IC50 )为 9.5 9× 10 -8mol/L ,明显高于H2 受体拮抗剂 (3.70× 10 -5mol/L)。结论 兰索拉唑对兔离体壁细胞组胺诱导的酸分泌具明显的抑制作用 ,效果优于西咪替丁 ;

Objective Lansoprazole (Takepron Takeda. Co.) has been extensively used in the treatment of acid related diseases. As there is no report about the relation between the effects of anti secretion drugs and parietal cells, recently a model of parietal cells has been established in our laboratory. The anti secretory effect of lansoprazole and cimetidine was compared in vitro with this new model. Methods The parietal cells were isolated with the method of cell elutriation and continuous density gradient centrifugation. The effect of cimetidine and lansoprazole on histamine induced acid secretion in rabbit parietal cells was investigated by accumulation of 14 C aminopyrine. Results The purity and viability of isolated parietal cells were >80% and 95%, respectively. Both of them significantly inhibited histamine stimulated (10 -4 mol/L) acid secretion, but IC 50 was quite different. IC 50 of cimetidine was 3.70×10 -5 mol/L, and that of lansoprazole was 9.59 ×10 -8 mol/L. Conclusions Lansoprazole is much more effective than cimetidine in vitro in inhibition of histamine stimulated acid secretion of rabbit parietal cells. This study developed a model for the study of acid inhibition drugs.