反义胰岛素样生长因子Ⅱ与胞壁酰二肽联合抗肝癌实验研究

Study of combination therapy with antisense insulin like growth factor Ⅱ and MDP in the treatment of hepatocellular carcinoma

目的 探讨反义胰岛素样生长因子Ⅱ (反义IGF Ⅱ )与胞壁酰二肽 (MDP)联合抗肝癌作用。方法 化学合成人IGF Ⅱ 5′端部分cDNA片段 ,克隆于真核表达载体 pcDNA3 ;将反义IGF Ⅱ、MDP包裹于脂质体中 ,同时转导入体外培养的HepG2 人肝癌细胞株中、注射于裸鼠皮下人肝癌移植瘤内 ,观察两者联合应用对肝癌细胞生长的影响。结果 反义IGF Ⅱ与MDP同时转导入体外培养的HepG2 人肝癌细胞株后 ,细胞增殖处于停滞状态 ,同时注射于裸鼠皮下人肝癌移植瘤内 ,5只裸鼠中有 2只肿瘤完全消散。结论 反义IGF

Objective Effects of hepatocellular carcinoma of antisense insulin like growth factor Ⅱ (antisense IGF Ⅱ) combined with muramyl dipeptide (MDP) was studied. Methods A cDNA fragment of human IGF Ⅱ was synthesized and inserted into a eukaryotic expression vector of pcDNA 3 (antisense IGF Ⅱ ). Antisense IGF Ⅱ and MDP encapsulated with liposomes were introduced into HepG 2 human hepatocellular carcinoma cells and were injected into subcutaneous tumors generated in nude mice. The growth of HepG 2 cells transfected with antisense IGF Ⅱ and MDP, and the subcutaneous tumors injected with antisense IGF Ⅱ and MDP were observed. Results The growth of HepG 2 cells was blocked completely, and the subcutaneous tumors disappeared in two of five nude mice. Conclusions The combination therapy of antisense IGF Ⅱ and MDP is effective in the treatment of hepatocellular carcinoma.