大鼠血浆中Alzheimer病相关分子的检测

Determination of Alzheimer Disease-related Molecules in Peripheral Blood of Rats

为检测外周血液中阿尔茨海默病 (Alzheimer病 ,AD)相关分子的表达特性 ,寻找 AD早期诊断的外周标记分子 ,采用了以下方法 :1外周血液中血浆、血小板和白细胞的制备 ,2 Western印迹技术 ,3扫描 -图像定量分析技术。结果显示在 SD大鼠外周血浆中可检测到磷酸酯酶 (PP) - 1和 PP- 2 A的表达 ,而 tau蛋白的免疫反应为阴性。提示外周血可用作 AD磷酸酶表达异常的研究样品 ,但不能用来检测 AD患者

To investigate the peripheral molecular marker for early diagnosis of Alzheimer disease (AD), the peripheral blood of the rats was observed by using the following methods: ① preparation of plasma, platelet and leukocyte in peripheral blood; ② Western blotting; ③ quantitative scanning image analysis. The results showed that protein phosphatase (PP) 1 and PP 2A were detected in rat plasma and no tau protein was demonstrated in various fractions of rat peripheral blood with different antibodies at the same condition. It was suggested that plasma is a potential peripheral sample for measuring the expression level of PP 1 and PP 2A. And tau protein may not exist in peripheral blood and can't be used as a peripheral marker for the diagnosis of AD.