武汉籍汉族类风湿关节炎患者HLA—DRB1＊04、＊01基因分型和临床相关性研究

A Study of Association between the HLA-DRB1 * 04,* 01 Alleles Genotyping and Rheumatoid Arthritis from Wuhan Han Nationality

为探讨人类白细胞抗原 (HL A) - DRB1 * 0 4、* 0 1基因与武汉籍汉族类风湿关节炎 (RA)的相关性。采用聚合酶链反应 -序列特异性引物法 (PCR- SSP) ,对 78例 RA患者和 12 6例健康者的 HL A- DRB1 * 0 1、* 0 4基因型进行检测。结果显示 RA患者 DR4基因频率为 5 2 .5 % ,对照组为 2 1.6 % ,两组有显著性差异 (P<0 .0 1) ,DR1 基因频率两组无差异 (P>0 .0 5 )。RA患者 HL A- DR4的主要亚型为 DRB1 * 0 40 5 (32 .1% )与对照组 (6 .3% )有显著性差异 (P<0 .0 1)。DR4阳性的 RA患者的关节压痛数、关节肿胀数、类风湿因子 (RF)阳性率、关节侵蚀性病变明显高于 DR4阴性的患者 (P<0 .0 1)。提示 HL A- DR4基因与 RA患者易感性及疾病严重性相关 ,其主要亚型为 DRB1 * 0 40 5、 DRB1 * 0 40 1。

To explore the association between the HLA DR gene and rheumatoid arthritis (RA) in Wuhan in Han nationality of China, the method of DNA amplification with sequence specific primers (PCR SSP) was used to determine HLA DRB 1 *01, *04 genotype in 78 patients with RA and 126 healthy controls from Wuhan of the Han nationality. The frequency of DR 4 was 52.5 % in RA patients and 21.6 % in the healthy controls ( P <0.01). The frequency of DR 1 revealed no difference between the two groups. The DRB 1 *0405 accounted for 32.1 % of DR 4 + in RA patients and 6.3 % of DR 4 + in controls ( P <0.01). According to the tender joint numbers, the swollen joint numbers, RF + ration, joint erosion, the RA patients of DR 4 + were higher than those of DR 4 - ( P <0.05). It was showed that the susceptibility and severity of RA was highly associated to the HLA DR 4 ,mainly the DRB 1 *0405, *0401. HLA DR 4 could be taken as a predictive marker for disease severity and a useful prognostic maker in the patients with RA.