抗人尘细胞单克隆抗体用于低SiO_2粉尘所致尘肺的诊断研究

Studies on diagnosis of low-SiO_2 pneumoconiosis with monoclonal antibody against human dust cell

目的 研究抗人尘细胞单克隆抗体 (McAb)用于低SiO2 粉尘所致尘肺病人的特异性及灵敏度检测。扩大抗人尘细胞McAb在尘肺诊断中的应用范围。方法 用人体巨噬细胞体外加SiO2 培养、提纯抗原免疫BALB/C小鼠获取致敏的小鼠脾细胞与骨髓瘤细胞融合、克隆化 ,筛选出两株分泌抗人尘细胞McAb的杂交瘤细胞 ,用其分泌的McAb分别测定了 138例各期尘肺病人的血清 ,其中矽肺 5 8例 ,电焊工尘肺 33例 ,煤工尘肺 39例 ,石棉肺 8例以及 84例 0 +观察者、5 4例非接尘作业的正常人 ,32例肺科病人。结果 矽肺、低SiO2 尘肺与抗人细胞McAb反应的均值无显著性差异 (P >0 0 5 ) ,各类尘肺与正常人之间有明显的界限值分别为 ( 1 77± 0 41)g/L ,( 0 98± 0 34 )g/L。Ⅰ、Ⅱ、Ⅲ期各类尘肺间无明显的剂量反应关系。结论 机体接尘后所产生的异性蛋白具有同源性 ,相对抗人细胞McAb是同一种抗原。抗人细胞McAb不仅可用于矽肺的检测诊断 ,同样也可用于低SiO2

Objective To study the sensitivity and precision on diagnosing low SiO 2 pneumoconiosis with anti human dust cell monoclonal antibody (McAb) and the expansion of its application range. Methods To purify antigen by vitro cultivating human macrophage with SiO 2 and immunized BALB/C mouse. To fuse the SP2/O mouse myeloma cells and the splenocyte of immunized BALB/C mouse. Two hybrids cell lines secreting anti human dust cell McAb were established by clone.Serum in 58 silicosis, 33 welders' pneumoconiosis, 39 coal workers' pneumoconiosis, 8 asbestosis, 84 0 + object of observation, 54 non exposed normal and 32 cases of pulmonary disease were measured by this McAb.Results It did not show significant difference between silicosis low SiO 2 pneumoconiosis and anti human dust cell McAb ( P>0.05 ). There was a significant line between every pneumoconiosis and non exposed normal (1.77±0.41) g/L, (0.98±0.34) g/L but no close response relationship could be found between every stage's pneumoconiosis and anti dust cell McAb. Conclusion Anti human dust cell McAb is not only used for diagnosis of silicosis but also used for diagnosis of low SiO 2 pneumoconiosis.