放疗对人宫颈癌组织的增殖细胞核抗原(PCNA)和c-fos表达的影响

Influence of radiotherapy on expression of the proliferating cell nuclear antigen(PCNA) and c-fos in human cervical cancer

目的 探讨照射前、后人宫颈癌组织增殖细胞核抗原 (PCNA)和c fos表达的变化。方法 在多聚甲醛固定的活检癌组织冰冻切片上 ,进行PCNA和Fos的免疫组织化学染色。结果 放疗前的宫颈癌组织内 ,多数癌细胞呈PCNA免疫染色阳性 ;放疗后 ,多数癌细胞无PCNA染色 ,但间质细胞的PCNA免疫染色明显升高 ,大量间质细胞则呈中等或强阳性染色。放射治疗前的宫颈癌组织内 ,多数癌细胞呈Fos免疫染色阳性 ;放疗后的宫颈癌组织Fos免疫染色显著降低 ,多数癌细胞无Fos染色 ,但放疗前、后间质细胞的Fos免疫反应无明显变化。结论 照射抑制宫颈癌细胞的PCNA和c fos表达的同时 ,诱导间质细胞的PCNA表达。提示PCNA和c fos的表达状态可作为放射治疗中评价宫颈癌细胞增殖和间质组织修复的分子指标之一。

Objective\ To investigate changes of proliferating cell nuclear antigen(PCNA)expression in human cervical cancer following irradiation. Methods\ Immunohistochemical staining for PCNA was performed in frozen sections of formalin fixed cervical cancer biopsy tissues. Results\ The majority of the cancer cells showed PCNA immunoreactivity before irradiation.Following irradiation(30-40 Gy/15-20 f)PCNA immunopositive staining was hardly detectable in most of the cancer cells.The PCNA immunoreactivity,however,increased after radiotherapy,and moderate or heavy immunopositive staining for PCNA was seen in irradiated mesenchymal tissue cells.On the other hand,after irradiation Fos immunoreactivity decreased remarkably,and Fos immunopositive staining was hardly detectable in most of cancer cells.No obvious change in Fos immunoreactivity,however,was seen in mesenchymal connective tissue following irradiation. Conclusion\ Irradiation inhibits PCNA and c fos expression in cervicval cancer cells whereas it induces the expression of PCNA in mesenchymal tissue cells.The present results suggest that expression of PCNA and c fos may be regarded as a molecular marker for evaluating the cancer cell proliferation and mesenchymal tissue repair during radiotherapy of human cervical cancer.\;