小鼠体外培养囊胚辅助孵化的初步研究

A Preliminary Study on Assisted Hatching of Mouse in vitro Cultured Blastocysts

目的 :探索体外培养和辅助孵化技术对体外发育小鼠囊胚孵出率的影响。方法 :将昆明白种小鼠的 2～ 4-细胞胚于体外培养到囊胚 ,观察比较 :(1 )体外培养囊胚与体内发育囊胚的自然孵化率 ;(2 )体外培养囊胚经辅助孵化后与未行辅助孵化 (对照组 )的体外孵化率。结果 :(1 )体外培养囊胚与体内发育囊胚的自然孵化率分别为 75.4%和 96 .2 % ;有显著差异 (P<0 .0 0 1 )。 (2 )辅助孵化与对照组的体外孵化率分别为 97.7%和 74.0 % ,有明显差异 (P<0 .0 5)。结论 :体外培养对囊胚的孵化有不利影响 ;

Objective: To study the influence of in vitro culture and assisted hatching on the hatching efficiency of mice blastocysts cultured in vitro. Methods:2 and 4 cell stage mouse embryos of Kun Ming White Strain were cultured in vitro to blastocysts. To compare: (1) spontaneous hatched rates of blastocysts in vitro cultured and in vivo developed;(2) hatched rates of in vitro cultured blastocysts after assisted hatching and non assisted hatching. Results: (1) natural hatched rates were 75.4% and 96.2%, in vitro cultured and in vivo developed respectively, diference significant (P<0.001); (2) in vitro hatched rates of assisted hatching group and control group were 97.7% and 74.0% respectively, significantly different (P<0.05). Conclusion: In vitro culture may influence the hatching of blastocyst; And assisted hatching can make it more efficient .