肾病综合征患儿白细胞介素10在外周血单个核细胞中基因表达的研究

Interleukin-10 gene expression of PBMC from children with nephrotic syndrome

目的 探讨白细胞介素 10 (IL 10 )在小儿原发性肾病综合征 (INS)中的变化。方法 应用逆转录 多聚酶链反应 (RT PCR)及ELISA法分别检测了 3 0例健康儿童和 5 2例原发性肾病综合征患儿急性期及缓解期外周血单个核细胞 (PBMC)在植物血凝素 (PHA)刺激下IL 10mRNA和蛋白水平的变化。结果 ( 1)原发性肾病综合征 (单纯性及肾炎性 )患儿急性期IL 10mRNA与蛋白水平较正常对照组显著升高 (P分别 <0 0 5及 0 0 1) ;而缓解期与正常对照组相比较无显著差异 (P均>0 0 5 )。 ( 2 )肾炎性肾病急性期IL 10mRNA、蛋白水平均显著高于单纯性肾病综合征 (P均 <0 0 1)。结论 原发性肾病综合征患儿存在Th1/Th2细胞免疫功能的失衡 ,IL 10在其发病中可能具有一定的保护作用。外周血单个核细胞IL 10水平可作为观察病情活动状态、判断临床类型的一个免疫学指标。

Objective To investigate the changes of interleukin 10(IL 10) in children with idiopathic nephrotic syndrome(INS).Methods The production of IL 10 protein was determined by ELISA and the expression of IL 10 mRNA was measured by reverse transcription polymerase chain reaction(RT PCR) in phytoheamagglutinin(PHA) stimulated peripheral blood mononuclear cells (PBMCs) in 30 healthy children and 52 children with INS during their acute phase and remission stage,respectively.Results (1)The expression of IL 10 mRNA and protein were significantly increased in patients with idiopathic nephrotic syndrome in acute symptomatologic phase compared with controls( P <0 05,0 01 respectively),but no significant difference was found in remission stage ( P >0 05).(2)The expression of IL 10 protein and mRNA were significantly evaluated in patients with nephritic nephrosis than patients with simple nephrosis( P <0 01 for both).Conclusion The results suggest that there exist the disorder of Th1/Th2 cellular immunity in patients with INS.IL 10 may be involved in the pathogenesis of INS.The expression of IL 10 in PBMCs may serve as a mark to predict the activation of INS and estimate the clinical types of INS.