荧光PCR技术检测HBV-DNA的初步研究

Pilot Study on Detection of HBV-DNA by FQ-PCR

目的:研究荧光PCR技术在临床的应用价值。方法:选择90份HBV-ELISA阳性标志物血清进行HBV-DNA的检测,从血清中提取DNA,运用iCycler-PCR软件进行荧光PCR扩增,对结果进行分析。结果:HBsAg-HBeAg-HBcAb组阳性率为96.7％,HBsAg-HBeAb-HBcAb组阳性率80％,HBsAg-HBcAb组阳性率55％。结论:荧光PCR技术是一种良好的检测HBV-DNA的技术,但尚不能作为乙型肝炎治愈与否的诊断标准。

To study the clinical significance of the technique of quantitative fluorescence-polymerase chain reaction (FQ-PCR). Methods: Ninety serous samples were selected from the patients with positive HBV results by ELISA and amplified by FQ-PCR. Their results were analyzed with the software of iCycler-PCR. Results: The percent of HBsAg-HBeAg-HBcAb having positive results was 96.70 % , while the positive rate of HBsAg-HBeAg-HBcAb was 80.00 % , and 55.00 % of the patients had a positive sign of HBsAg-HBcAb. Conclusion: FQ-PCR is a good quantitative method to detect HBV-DNA, but it is not a diagnostic standard to identify whether the hepatitis B is cured or not.