肠道细菌易位途径的实验研究

AN IMMUNOHISTOCHEMICAL STUDY ON THE ROUTE OF TRANSLOCATION OF BACTERIA FROM THE GUT

采用给无菌大鼠管饲O_(111)B_4型埃氏大肠杆菌的方法,用抗O_(111)B_4型抗血清PAP技术在石蜡切片上定位显示了肠粘膜和其它脏器中该细菌的踪迹。发现了阳性染色颗粒(可能为细菌碎片和内毒素)和杆菌侵入肠粘膜基底部的病变并广泛分布于肠系膜淋巴结,肝、脾以及肺脏的血管内皮细胞和吞噬细胞中。该观察结果提示大量细菌和毒素可经肠粘膜深部病变进入血循环,它们在全身的播散与吞噬细胞功能有关。

Indigenous infection induced by bacterial translocation from the gut has become a major cause leading to systemic septic state or even multiple organ failure syndrome (MOF). In order to examine the route of the bacterial translocation, including the lesions where bacteria penetrate the barrier of intestinal mucosa and the fate of the translocated bacteria in other organs, we tube-fed E.Coli O111 B4 to germ-free rats. An immuno-histochemical method, with anti E.Coli O111 B4 seta PAP technique, was used on paraffin sections to trace the organisms in intestinal mucosa and other organs.Positively stained particles (bacterial debris and endotoxin) and bacilli were found in the basal portion of intestinal mucosa and also distributed extensively in the endothelial cells of blood vessels as well as in the phagocytes of MLNs, liver, spleen and lungs. It was suggested that a large number of bacteria have penetrated intestinal mucosa in deeper lesions, and their systemic dissemination was associated with the function of phagocytes and mainly through the blood vessels.