镉化物对人胚肺成纤维细胞转化作用的研究

THE EFFECT OF CADMIUM ON THE TRANSFORMATION OF HUMAN EMBRYO LUNG FIBROBLAST CELLS

本文应用人胚肺成纤维细胞转化试验,对氯化镉的致癌性进行研究。结果表明:氯化镉在浓度0.004～0.04μM/ml范围之内,可使人胚肺细胞形态发生改变,出现明显的转化灶,细胞与刀豆蛋白A的凝集作用加强,细胞失去接触抑制特性,获得在软琼脂培养基中生长能力及细胞在软琼脂中集落形成率增高,细胞的染色体数目出现变化,增多或减少,及结构的畸变、断裂、断片等。细胞的寿命明显延长。本文通过分析各种指标,较完整的反映了氯化镉使细胞恶性转化的多阶段的转化过程,证实了体外培养的细胞转化和肿瘤的演化,与它在体内的类似物的作用一样,是一个具有多步骤的变化过程。通过实验,初步建立了氯化镉致恶性转化体外培养的实验模型、为进一步研究癌变的分子基础提供条件。

The cultural HEL fibroblast cells transformation test was done in or-der to research the carcinogenic nature of CdCI_2, and to observe some bio-logical characters of the transformed cells. The results showed that themalignant transformation of HEL cells may be induced by CdCI_2. In cer-tain concentration limit the treatment times and the concentration ofCdCI_2 were proportional to the degree of cell transformation. The HEL cells treated by CdCl_2 had great change in their shape. Theybecame clear transformation foci and no longer had anchorage dependence.ConA agglutination was enhanced, the dependence of density of cell grow-th became lower. Cells saturated density decreased apparently. The lifetime of cell growth apparenty became longer. The analysis of transforma-tion of cell chromosome showed that allopolyploidy was higher than thatin international upper limit standard. This study shows that CdCI_2 mayput HEL cells to transform. CdCI_2 may be a potential carcinogen.