用显示非特异性酯酶的半透膜技术对小鼠胸腺基质细胞的观察

THE THYMIC STROMAL CELLS OF THE MICE AS DEMONSTRATED BY SEMIPERMEABLE MEMBRANE TECHNIQUE FOR NON-SPECIFIC ESTERASE

成年BALB/c小鼠胸腺,新鲜组织恒冷箱切片,用半透膜技术显示非特异性酯酶。由于该技术能保存全部酶活性,故可显示各类基质细胞及其分布。上皮网状细胞、胸腺哺育细胞及交错突细胞的酶活性呈弱阳性,巨噬细胞呈强阳性。胸腺T细胞呈微弱阳性或阴性。皮质的上皮网状细胞形成海绵状支架;髓质上皮网状细胞可分为低酶活性和高酶活性细胞两种类型,后者可呈灶状分布。皮、髓质内均可见巨噬细胞,皮-髓交界区尤多,并可见与胸腺T细胞形成玫瑰花环状。同时用大鼠抗小鼠基质细胞的单克隆抗体进行了免疫组织化学观察。本文结果有助于在光镜下研究胸腺微环境的结构和功能。

Fresh cryostat sections from the thymus of BALB/c adult mice were processed with semipermeable membrane technique for demonstration of non-specific esterase (NE). The various cell types and the pattern of their distribution may be showed, because this technique may preserve the total enzyme activity of cells. The NE activity of epithelial reticular cells (ERC), thymic nurse cells (TNC), and interdigitating cells (IDC) are lower, but macrophages (Mφ) show a high activity. Cortical ERCs appear as a sponge-like network. Medullary ERCs may be divided into two cell types, i. e. low and high activity cells. The distribution of the latter shows foci-like pattern. Mφ present in both the cortex and medulla, in the cortico-medullary border they are prominent and may form rosettes-like structure with thymocytes. The thymus was also studied with immunohistochemical method using the monoclonal antibody of rat-anti-mouse thymic stromal ceils (MTS-6). This observation enable studies on the type of thymic stromal cells formed thymic microenvironment under LM.