大鼠肺泡巨噬细胞类型的形态学研究

A MORPHOLOGICAL STUDY ON TWO TYPES OF ALVEOLAR MACROPHAGES FROM NORMAL RAT LUNG

本实验用相差显微镜、微分干涉差显微镜、扫描电镜和透射电镜,对肺泡巨噬细胞进行了观察;并用显微分光图像分析仪测定了细胞的酸性磷酸酶(AcP)、乳酸脱氢酶(LDH)和琥珀酸脱氢酶(SDH)含量。本文的结果表明,肺灌洗液中存在着两型不同的肺泡巨噬细胞。在相差显微镜下,呈现为明细胞和暗细胞;在微分干涉差显微镜和扫描电镜下,则呈现为球形细胞和扁平细胞。在扫描电镜下,球形细胞伸出较多的丝状突起,扁平细胞伸出较多的板状突起。在透射电镜下,球形细胞比扁平细胞含有更丰富的溶酶体。用定量细胞化学测定两型细胞的AcP、LDH、SDH含量,球形细胞明显高于扁平细胞。

Alveolar macrophages (AM) were studied by means of phase contrast microscope, differential interference contrast microscope, SEM and TEM. The content of AcP, LDH, SDH of the AM was measured by MPV3-TSA microspectrophotometerimage analytical instrument. Under phase contrast microscope, two different types of AM can be distinguished, i. e, the light cells and the dark cells. The two types of cells are spherical and flat in shape under differential interference contrast microscope. The observation with SEM and TEM showed that the spherical cells possess more filopodia and more lysosomes in cytoplasm, but the flat cells possess more lamellar podia. Quantitative cytochemistry demonstrated that the content of AcP, LDH, SDH in spherical cells are much more than that of flat cells. The results suggest that there are two types of alveolar macrophages exist in normal rat lung.