周围突分支至肺与体表的降钙素基因相关肽免疫反应阳性感觉神经元

THE BIFURCATING INNERVATION OF THE LUNG AND BODY SURFACE FROM PERIPHERAL PROCESS OF CGRP-IMMUNOREACTIVE NEURONS IN PRIMARY SENSORY GANGLIA

将碘化丙啶(PI)注入大鼠肺内,双苯甲亚胺(Bb)注入右侧2～6肋间神经或/和双侧外耳皮肤。于双侧C_1～T_(11)背根神经节、迷走神经上、下节发现PI标记细胞;右侧T_(1～8)背根节、双侧迷走神经上、下节发现Bb标记细胞;PI/Bb双标细胞见于右侧T_(1～8)背根节及迷走神经上、下节。同一切片用PAP方法证实,部分PI/Bb双标细胞为降钙素基因相关肽免疫反应阳性。

Propedium iodide(PI) was injected into the lung of adult rats, and bisbenzamide(Bb) was injected into bilateral T_(2-6) intercostal nerves and/or the subcutis of bilateral external ears two days later.PI labeling neurons were found in C_1-T_(11) dorsal root ganglia, bilateral vagal superior and inferior ganglia. Bb labeling cells were observed in right T_(1-8) dorsal root ganglia and vagal sensory ganglia. By means of immunohistochemical PAP method for demonstration of CGRP consecutively, we found some double-labeled neurons were positive for this bioactive peptide.