比较研究人正常、增生性胃粘膜和胃癌时对酶标凝集素的反应

COMPARATIVE STUDYING THE REACTION OF GLYCOCONJUGATES IN HUMAN GASTRIC MUCOSA HYPERPLASTIC MUCOSA AND CARCINOMA WITH HRP-LECTINS

本文用酶标凝集类,对35例胃手术病人的正常胃粘膜、增生性胃粘膜、小肠化生和胃癌等与凝集素的反应,在光镜上作了比较观察。结果表明正常胃粘膜的上皮细胞对PNA、DBA和ConA均为阴性。而胃小凹上皮PNA和DBA则为阳性。增生肥大的胃粘膜、上皮细胞和胃小凹上皮对PNA和DBA均匀阳性反应,其染色强度在胃小凹上皮有所增强。化生的胃粘膜,PNA阳性,但DBA阴性。胃腺的主细胞仅对ConA染色。壁细胞能选择性地与DBA结合,有的壁细胞也能与PNA结合。癌细胞与凝集素反应的能力比增生性粘膜减弱,可能是由于癌细胞缺少糖苷化作用酶的关系,

The samples of gastric mucosa and candcarcinoma were obtained from 35 patients and were examined by light microscope with three kinds of labelled lectin to detect and to compare their reaction between them.The results indicated that the normal gastrice epithelia remained unstainee by DBA and PNA, but the foveolar epithelia were stained intensively for PNA and DBA. In the hyperplastic mucosa, the surface epithelia and foveolar cells enhanced lectin staining by PNA and DBA.The cheif cells in mucosa were only stained by ConA, while the parietal cells displayed a high grade binding with DBA. and less staining with PNA.The staining intensity of tumor cells with PNA and DBA was less reaction than that of hyperplastic or foveolar epithelia, and the tumor cells had differnt affinity for lectin staining.