氟氢松醋酸酯对人体皮片郎格罕氏细胞的影响

EFFECTS OF FLUOCINOLONE ACETONIDE ON THE LANGERHANS CELLS IN HUMAN SKIN GRAFTS

已证明郎格罕氏细胞(LC)在皮肤移植排斥反应中起重要作用,若能在皮片移植前选择性减少LC,就可延长皮片存活期。本文选免疫抑制剂氟氢松醋酸醇(FA)体外处理人体皮片,用酶组化,免疫组化及电镜观察FA对皮片中LC的影响。结果显示,经FA处理后的皮片中LC密度减小,细胞形态发生退行变化。我们推论,用FA体外处理皮片。

It has been proved that Langerhans cells (LC) play an key role in the immunologic rejection of skin allografts.We use an immunosuppressive agent floucinolone acetonide (FA) to treat human skin grafts in vitro and observe its effects on the LC of the grafts. The results show that the numbers of the ATPase and Ia positive LC decrease significantly in the FA treated grafts, and in morphology, some deteriorative changes occur in the LC.We infer that using FA. to treat human skin graft is possible to oecome one of the effective methods of prolonging allografts survival time in clinic