昆明山海棠抗生育作用的形态学研究——Ⅰ.睾丸、附睾的组织化学观察

MORPHOLOGICAL STUDIES ON THE ANTIFERTILITY EFFECT OF TRIPTERYGIUM HYPOGLAUCUM: Ⅰ.HISTOCHEMICAL OBSERVATIONS ON THE TESTIS AND EPIDIDYMIS

本文应用组织化学方法探讨昆明山海棠(TH)对雄性大鼠睾丸,附睾的DNA、RNA.乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、ATP酶,葡萄糖-6-磷酸脱氢酶(G-6PDH)等的影响。成年雄性SD大鼠用昆明山海棠根50%乙醇提取物灌胃给药,剂量1.0g/kg/日,每周六次,共6周,所有用药鼠均丧失生育力。用药鼠附睾管腔内精子减少或完全消失,并可见少量脱落和退化的生精细胞(主要是精子细胞)。附睾头、尾部管腔内精子大部分呈断头、卷尾等畸形。精子LDH.SDH活性明显下降。精子细胞及残余体RNA凝集成块,而精原细胞、精母细胞,支持和间质细胞及附睾各段上皮细胞的形态和组织化学均未见明显改变。本实验表明,TH主要影响精子细胞RNA的功能及精子糖代谢,而对其它生精细胞无明显作用。

In adult male SD rats, an alcoholic extract of the title plant was given orally at a dose of 1 g/kg/day for 5 weeks, when rats became infertile.At that time, the number of sperm in cauda epididymis was dramatically reduced, mostly with the head and tail separated.About 1/3 of tails were coiled.Exfoliated germ cells, minly spermatids and a few multinucleated macrospermatids, could be seen in the epididymal lumen.In the seminiferous tubules, RNA granules in spermatids were condensed.The activities of LDH and SDH of epididymal spermatozoa were dccreased, while those of ATPase and G-6-PDH were unaffected.