分娩后小鼠腹腔巨噬细胞的形态学观察及溶菌酶测定

MORPHOLOGICAL INVESTIGATIONS ON MACROPHAGES FROM POSTPARTURIENT MOUSE PERITONEAL EXUDATE AND MEASURING ON LYSOZYME

小鼠腹腔巨噬细胞(Mφ)于妊娠期间持续增加,尤以妊娠晚期和分娩后增加最为显著。用普通光显微镜、扫描电镜及透射电镜观察,分娩后小鼠腹腔Mφ的形态具有明显的不同,呈现出功能活跃的形态学特征。分娩后小鼠腹腔Mφ的吞噬能力、形成Mφ——自体胸腺细胞花环的能力都高于对照组,分娩后小鼠血清溶菌酶活性亦明显增高。试验结果表明分娩后小鼠腹腔Mφ处于极其活跃的机能状态。

There was a persistent increase in the number of the peritoneal macrophages throughout mouse pregnancy.There was a significant increase in the number of the peritoneal macrophages during the third trimesters of pregnancy and post-parturturient.Light microscope,scanning electron microscope, transmissional electron microscope were used to investigate the morphology of macrophages from mouse peritoneal exudate in post-parturient mouse.The macrophages from mouse peritoneal exadate exhibit obviously difference and activated morphological character.The activity of serum lysozyme and the ability of froming macrophage-thymocyte autorosettes in peritoneal macrophages from post-parturient mouse were obviously higher than normal mouse,These results show that the peritoneal,macrophages from post-parturient mouse exhibit the obviously activated functional and morphological changes.