致病性镰刀菌体外药敏试验研究

Antifungal susceptibility testing in vitro for pathogenic Fusarium sp

目的 探讨用于镰刀菌敏感的抗真菌药物 ,为临床治疗提供参考。方法 通过对镰刀菌的体外药敏试验 ,按照美国临床实验室标准化委员会推荐的M38 P方案 ,用微量稀释法测定 40株茄病镰刀菌及 6株串珠镰刀菌 ,对 7种抗真菌药物即两性霉素B、酮康唑、氟康唑、伊曲康唑、5 氟胞嘧啶、咪康唑、特比萘芬的敏感性。结果 40株茄病镰刀菌 :MIC5 0 :两性霉素B 4μg/ml、酮康唑 16 μg/ml、氟康唑 6 4μg/ml、伊曲康唑 16 μg/ml、5 氟胞嘧啶 6 4μg/ml、咪康唑 16 μg/ml、特比萘芬 0 .2 5 μg/ml,MIC90 :除两性霉素B及特比萘芬外 ,其余药物均与MIC5 0相同 ,两性霉素B 8μg/ml,特比萘芬 4μg/ml。 6株串珠镰刀菌的MIC值除两性霉素B、氟康唑与茄病镰刀菌MIC5 0相同外 ,其余各药均低于茄病镰刀菌MIC5 0。结论 特比萘芬MIC值较低 。

Objective To study the susceptible drugs for Fusarium , and provide references for clinical treatment. Methods 40 F.solani and 6 F.moniliforme isolates were examined for the susceptibility to 7 antifungal drugs : AmB, ketoconazoie, miconazole, itraconazole, fluconazole , 5 Fc, and terbinafin by broth microdilution of M38 P recommended by NCCLS. Results 40 F.solani :MIC50 AmB 4 μg/ml, ketoconazoie 16 μg/ml, fluconazole 64 μg/ml, itraconazole 16 μg/ml, 5 Fc 64 μg/ml, miconazole 16 μg/ml, terbinafin 0.25 μg/ml. MIC90: In addition to AmB and terbinafin , others antifungal agents were similar to MIC50, AmB 8 μg/ml, terbinafin 4 μg/ml. 6 F.moniliforme: except AmB and fluconazole were similar to MIC50 of 40 F.solani , others were lower than F.solani MIC50. Conclusion The MIC of terbinafin MIC is lower than that of others drugs, it could be used in the treatment of infections coused by Fusarium sp .