17-β雌二醇、孕酮对增生性瘢痕成纤维细胞转化生长因子-β_1合成的影响

Influnence of 17-β estradiol and progesterone on synthesis of transforming growth factor beta-1 in hyperplastic scar fibroblasts

目的 研究雌激素、孕激素对增生性瘢痕形成的影响。方法 体外培养增生性瘢痕成纤维细胞(HSFB) ,利用免疫组化及计算机图像分析系统测定转化生长因子 (TGF β1)含量。结果 较对照组 ,17 β雌二醇(17 βE2 )各组的HSFB胞浆内阳性信号明显加强 (P <0 .0 5 ) ;而孕酮 (P)各组均不明显 (P >0 .0 5 )。结论 在体外 ,17 βE2 可以明显促进增生性瘢痕成纤维细胞的TGF β1合成 ,而P的作用不明显。

Objective To study the influnence of estrogen and progestin on scar formation. Methods By culturing hyperplastic scar fibroblast(HSFB), we investigated TGF β 1 synthesis by immunohistochemical staining and image analysis. Results The detectable level of TGF β 1 in HSFB treated with 17 β E 2 was higher than that of the control significantly( P <0.05), but the one treated with progestin was not( P >0.05). Conclusion In vitro, 17 β E 2 can stimulate TGF β 1 synthesis in HSFB significantly.