中药皮肤剥脱剂对豚鼠皮肤损伤的实验研究

Cutaneous damage of guinea pigs induced by Chinese medicinal peeling

目的 为中药皮肤剥脱剂的临床应用提供实验依据。方法 将药物外用于豚鼠皮肤 ,观察不同时间、不同药物对豚鼠皮肤的损伤。结果 3d后中药皮肤剥脱剂组对豚鼠皮肤损伤达 0 .2 2± 0 .0 6mm(棘细胞和真皮乳头层 ) ;贝克 -戈登液组损伤达 0 .5 3± 0 .0 3mm(真皮网状层 ) ;杰森液组损伤达 0 .1±0 .0 2mm(角质浅层 ) ;低浓度中药皮肤剥脱剂组损伤达 0 .12± 0 .0 3mm(角质层 )。中药皮肤剥脱剂和贝克液组角质蛋白均出现凝固、坏死、真皮上中层胶原纤维发生重新排列 ,真皮网状层有中性粒细胞、巨噬细胞、淋巴细胞等炎性细胞浸润 ;基质对照组未见皮肤损伤。结论 中药皮肤剥脱剂优于低浓度中药皮肤剥脱剂和化学剥脱剂 ,既能控制深度 ,避免太深引起瘢痕 ,又能加速表皮死亡细胞或受损细胞的脱换率 ,促进真皮乳头层新生胶原蛋白的合成以及胶原纤维的重新排列 ,使真皮变厚 ,皱纹消失 。

Objective To provide laboratory data of Chinese medicine peeling for clinical application.MethodsThe experimental durgsexposed at different times was investigated to observe cutaneous damage in guinea pigs. Results Three days after the application of the Chinese drugs, the extents of damage of the skin was as follows: 0.22±0.06 mm in depth(in malpighian and dermis papillary layer) in NeFuji group; 0.53±0.03mm(in dermis reticular layer)in Baker Gorden liquid gruop;0.10±0.02 mm(in superficial stratum corneum layer)in Jessner liquid group;0.12±0.03 mm(in stratum corneum layer)in group of lower concentration NeFuji. In groups of Chinese medicinal peeling and Baker Gorden liquid, solidification and necrosis of corneum protein were noted and rearrangement of collagenous fiber in upper middle layer of derma took place. The layer of dermis reticulum was infiltrated by neutrophilic granulocytes, lymphocytes and macrophages. No skin damage was found in control group. Conclusions The Chinese medicinal peeling is superior to chemical peeling in terms of controlling the depth and avoidance of scar formation, acceleraes the metabolism of dead and injured cells of epidermis with thickening of the dermis layer and disappearance of wrinkles, and reaches the goal of tender skin.