卡介苗接种对高盐及肾动脉狭窄术诱导的高血压大鼠血压的影响(英文)

Effect of mycobacterium bovis bacillus calmette-guerin vaccination on arterial pressure in hypertensive rats

目的 :观察卡介苗接种对高盐负荷及肾动脉狭窄所致高血压的影响 ,为寻找防治人类高血压的新方法提供实验依据。方法 :以高盐食物喂饲及肾动脉狭窄术分别制作高盐性高血压 (SH)及肾性高血压 (RH)模型。高盐喂饲或肾动脉狭窄术后 4周 ,选择高血压大鼠及正常对照大鼠进行实验。所有大鼠腹部皮下接种卡介苗一次 ,接种前及接种后每周测定血压变化情况 ,同时每时段收集 2 4h尿样并以Greiss反应法进行一氧化氮终产物NO-2 及NO-3 (UNOx)的测定。 3周后大鼠分别取主动脉组织并用Westernblot法检测iNOS蛋白水平。结果 :卡介苗接种对正常大鼠血压无明显影响。但接种 1周时 ,能使高血压大鼠的血压明显下降。其中尤以卡介苗接种 +L -精氨酸作用明显。NO测定表明 ,卡介苗接种后体内NO生成明显增加。Westernblot结果证明卡介苗接种后 ,高血压大鼠主动脉血管组织中iNOS蛋白明显高于正常大鼠。结论

AIM: The present study was designed to examine the effect of Bacillus Calmette-Guerin (BCG) vaccination on blood pressure, nitric oxide (NO) production and iNOS expression in hypertensive rats. METHODS: Renal hypertension (RH) were made by renal artery stenosis in SD rats and the sodium induced hypertensive (SH) rats were made by feeding the rats with high sodium chloride diet (5 g NaCl/100 g food). After four weeks, the hypertensive animals were subjected to the experiment. All the rats were vaccinated with BCG (0 1 mL, i.d) and blood pressure were examined every week. Greiss reaction was used to measure the urinary NO excretion and Western blot was applied to probe the iNOS protein expression in aortic tissue. RESULTS: It was shown that one week after BCG vaccination, the blood pressure decreased significantly in hypertensive rats induced by NaCl-overloading and renal artery stenosis, but not in normotensive control rats. Furthermore, the hypotensive effect of BCG vaccination was enhanced by co-administration of L-arginine. A significant increase in NO production was observed in hypertensive rats. Also, Western blot showed BCG vaccination led to an obvious increase in iNOS expression in the aortic tissue of hypertensive, but not of normal control rats. CONCLUSION: BCG vaccination could lower the blood pressure of hypertensive rats through activation of iNOS/NO pathway. [