家兔脑膜炎双球菌感染性休克的肾上腺病变

The damages of adrenal gland in rabbits with neisseria memingococcal septic shock

目的 :探讨家兔被脑膜炎双球菌 (MC)攻击后肾上腺组织的病变特征。方法 :采用MC -A群菌珠 ,按 1× 10 11菌体 /kg体重剂量 ,清醒动物iv(模型组 ,n =10 )复制成动物模型 ;并以生理盐水为对照 (对照组 ,n =8)。给药前、给药后 6 0min、12 0min分别检查血浆TNF -α水平 ,12 0min后处死动物 ,取肾上腺做TNF -α细胞组化和超微结构检查。结果 :模型组髓质区内可见TNF -α阳性细胞、TNF -α阳性物质释放入髓质并引起髓质坏死。肾上腺皮质细胞无病变。血浆TNF -α浓度增高。结论 :MC攻击家兔后 ,可引起TNF -α在肾上腺髓质释放并引起病变 。

AIM: To investigate the damages of adrenal gland in rabbits infected with neisseria meningococci(MC).METHODS: 18 rabbits were injected with MC (1×10 11 organism/kg, iv, model group, n=10 ) or normal saline (control group, n=8 ).TNF-α was detected in baseline (before challenged), 60 min and 120 min after injection. 120 min after injection, TNF-α immunochemical localization of adrenal gland was detected with ABC methods by electron microscopy. RESULTS: In model group, TNF-α positive cells were located in medulla and TNF-α released into simus of medulla. The medulla damages were appeared but gland cells of cortex was normal. No damages were showed in control group. TNF-α value of plasm was elevated at 60 min and 120 min after injection compared with control group. CONCLUSION: TNF-α was released into medulla and resulted in medulla damages after MC challenging. Adrenal cortex didnt damaged in early phase of MC septic shock. [