非霍奇金淋巴瘤中细胞凋亡与细胞增殖的研究

Study on cell apoptosis and proliferation of non-Hodgkin's lymphoma

目的 了解非霍奇金淋巴瘤 (NHL)中细胞凋亡与细胞增殖间的关系 ,探讨两者在 NHL 发生与发展中的作用。方法 利用 Td T介导的 d UTP缺口末端标记 (TUNEL)法和 PCNA免疫组化技术原位检测 6 0例 NHL 中的细胞凋亡和增殖水平 ,计算凋亡指数 (AI)和增殖指数 (PI)。结果 B细胞性 NHL 中 ,随着恶性度增高 ,AI和 PI均增高 (P<0 .0 5 ) ,T细胞性 NHL 中高度恶性组 PI明显高于低度恶性组 (P<0 .0 5 ) ,而 AI在两组之间无显著性差异 (P>0 .0 5 )。AI和 PI呈显著正相关 (r=0 .70 4,P<0 .0 1)。结论 在 NHL 中细胞凋亡与增殖之间可能存在密切的联系 ,共同参与了 NHL 的发生和恶性进展。对细胞凋亡和增殖水平的检测可能有助于 NHL

Objective To investigate the relationship between cell apoptosis and proliferation and their roles in oncogenesis and progression of non Hodgkin's lymphoma (NHL).Methods TdT mediated dUTP nick end labeling (TUNEL) and immunohistochemistry of PCNA were used to detect in situ the apoptotic cells and the proliferative cells in 60 cases of NHL,then apoptotic index (AI) and proliferative index (PI) were calculated.Results AI of B cell type NHLs and PI of both type NHLs increased with of malignancy grade ( P <0.05),whereas there was no significant difference of AI between low grade and high grade of Tcell type NHLs ( P >0.05).A significant correlation was found between AI and PI (r=0.704, P <0.01).Conclusion Cell apoptosis may have a close correlation with cell proliferation in NHL and they may participate in oncogenesis and progression of NHL.Detection of cell apoptosis and proliferation may be helpful in judging malignancy grade and prognosis of NHL.\;