鼻咽癌染色体16q22-24遗传不稳定性的研究

Evidence of Genetic Instabilities on Chromosome 16q22-24 in Nasopharyngeal Carcinoma

目的：研究鼻咽癌染色体１６ｑ２２－２４的遗传稳定性。方法：用染色体１６ｑ２２－２４上的８对微卫星多态性标记分析 ５０例鼻咽癌的杂合性缺失（ｌｏｓｓ ｏｆ ｈｅｔｅｒｏｚｙｇｏｓｉｔｙ， ＬＯＨ）与微卫星不稳定性 （ｍｉｃｒｏｓａｔｅｌｌｉｔｅ ｉｎｓｔａｂｉｌｉｔｙ， ＭＳＩ）。结果：至少一个位点发生ＬＯＨ的肿瘤占４８％（２４／５０），ＭＳＩ的发生率为１８％（９／５０）。但这些变化均散在分布，未见高频共同缺失区和微卫星不稳定区；其变化在早期（Ⅰ／Ⅱ期）和晚期（Ⅲ／Ⅳ期）病人之间有显著性差异（Ｐ＜０．０５）。结论：染色体１６ｑ２２－２４区的遗传不稳定性的变化可能与鼻咽癌的发病有关，该区是否存在鼻咽癌相关基因有待进一步探讨。

Objective: This study was designed to evaluate the genetic stability on chromosome 16q22-24 in primary nasopharyngeal carcinoma. Methods: Tissue samples from fifty patients with nasopharyngeal carcinoma (NPC) tumors were examined by loss of heterozygosity (LOH) and microsatellite instability (MSI) analysis with a panel of 8 microsatellite polymorphic markers distributed along the chromosome 16q22-24. Results: LOH was observed at one or more loci in 24 cases (48% ) and the prevalence of MSI was detected in 9 cases (18% ). But the genomic alterations scattered along the region, neither common deletion or instability region was found. Conclusions: The status of genetic stability in chromosome 16q22-24 suggests that the genomic alterations on chromosome 16q22-24 may be involved in the development of NPC.