胎盘滋养细胞表面FasL的表达异常与妊娠高血压综合征的关系

The Relationship Between PIH and Abnormal Expression of FasL on the Human Placental Trophoblasts

目的 通过比较正常孕妇与妊娠高血压综合征 (简称妊高征 )患者胎盘滋养细胞表面Fas L(Fas配体 )表达 ,进一步从分子免疫学角度探讨妊高征的免疫病理机制。 方法 用特异性的Fas L 抗体对中、重度妊高征患者胎盘滋养细胞表面 Fas L 进行免疫组化染色 ,并通过高清晰彩色病理免疫组化测量系统对其定量分析 ,与同孕周的正常孕妇进行比较。 结果 妊高征组胎盘滋养细胞表面 Fas L 表达面积 [(6 9.6 2 8± 19.10 3) μm2 ]明显低于正常组 [(97.46 1± 10 .5 17) μm2 ],两者间差异有非常显著意义 (P<0 .0 1)。妊高征组胎盘滋养细胞表面 Fas L 表达强度 ,包括平均光度 (0 .117±0 .0 2 2 )及积分光度 (14.36 7± 3.15 6 )也明显低于正常组 (分别为 0 .145± 0 .0 15与 18.5 88± 3.897) ,两者间差异有非常显著意义 (P<0 .0 1)。 结论 胎盘滋养细胞表面 Fas L 表达减少 ,导致母胎间的免疫耐受的破坏 ,引起异常的免疫反应可能是妊高征发病的重要机制。诱导 Fas L 的产生或调节母胎间的免疫耐受 ,将为临床治疗妊高征提供新的方向。

Objective To study the immunol pathological mechanism of PIH through the expressron of FasL of placental trophoblasts. Methods Immuno histochemistry were used to detect the expression of FasL of placental trophoblasts on moderate and severe PIH patients, HPIS was used to determine the quantity of FasL. The expression of PIH group was compared with that of the normal control group. Result The scale of FasL expression on PIH group (69.628 ±19.103 μm 2 ) was significantly lower than that of control group(97.461±10.517 μm 2), P <0.01. The strength of FasL expression on PIH group, including an average brightness 0.117±0.022 and integral brightness 14.367±3.156 were also significantly lower than that of control group, (0.145 ±0.015, 18.588±3.897 respectively), P <0.01. Conclusions Abnormal expression of FasL on placental trophoblasts, which destroy the immunologic tolerance between the mother and the fetus, may be one of the important mechanism of the etiology of PIH. To induce the expression of FasL or to recover the immunology tolerance would be a new way of clinical treatment on PIH.