伯氏疟原虫氯喹敏感株和抗性株感染小鼠肝、肾、肺超微结构的比较

Comparing the ultrastructures of liver, kidney and lungs of ICR mice infected with chloroquine-sensitive and chloroquine-resistant strains of Plasmodium berghei

目的 :研究伯氏疟原虫 (Plasmodium berghei)氯喹抗性 (RC)株与敏感 (N )株致病力和宿主对不同虫株感染反应的差异。 方法 :比较伯氏疟原虫 RC株和 N株感染 ICR小鼠后期 ,肝、肾、肺等重要脏器超微结构的病理学改变。 结果 :N株感染后期 ,肝血窦、肾间质和毛细血管、肺泡毛细血管含有较多的感染疟原虫红细胞 ,肺泡壁毛细血管内皮细胞有较多的突起与疟原虫感染红细胞相接触。较多的肝细胞、肾小球旁细胞、肾小管上皮细胞、肺泡 型和 型细胞出现膜破溃 ,细胞器和胞质流失等不可逆的坏死性病变 ,一些肝血窦、肾小管和肺泡内可见多量的细胞碎片。 RC株感染后期 ,肝血窦、肾间质充血 ,含有较多的感染疟原虫红细胞。肝细胞、肾小管上皮细胞的病变以线粒体肿胀为主的变性 ,可见肝脏部分变性肝细胞出现恢复状态 ,肾小球毛细血管内皮细胞下、基膜、系膜区可见电子致密物沉着 ,肺泡隔内可见较多的单个核细胞。 结论 :N株毒力较强 ,对宿主肝、肾和肺细胞超微结构的损害为不可逆的坏死性病变 ,呈急性变质性炎症过程 ;而 RC株毒力明显减弱 ,对宿主肝、肾和肺细胞超微结构的损害以变性为主 。

Objective: To investigate virulence differences of the malaria parasites and responses of their hosts to the malaria infection between chloroquine sensitive (N) and resistant (RC) strains of Plasmodium berghei . Methods: Ultrastructural pathological alterations of liver, kidney and lungs of ICR mice infected with the N or the RC strains were investigated. Results: Congestion and more parasite infected red blood cells in hepatic sinusoids, renal interstitium, capillaries of the liver, kidney and lungs in late period of the infection with the N strain were observed. Necrosis of hepatic cells in liver, juxtaglomerular cells and epithelial cells of renal tubules in kidney, type Ⅰ and type Ⅱ alveolar cells in lungs occurred. Membranes of these cells were broken, resulting in cytoplasm missing and mitochondria swelling infected with the N strain. While degeneration of some hepatic cells of liver, epithelial cells of renal tubules, alveoli cells of lungs appeared during late period of the infection with the RC strain of P. berghei . It was observed that some hepatic cells recoved, the glomeruli basement membrane and mesangial cells contained electron dense deposits,alveoli septa were undergoing the proliferation and infiltration of lymphocytes, macrophages and neutrophilic granulocytes. Conclusion: The N strain is more virulent than the RC strain of P. berghei . Ultrastructures of the liver, kidney and lungs of the mice infected with the N strain show cellular necrosis, while those infected with the RC strain mainly reveal cellular degeneration.