摘要
目的 :探讨研制检测乙型和丙型肝炎病毒的基因芯片。方法 :以 HBV、HCV高度保守的基因片段为探针 ,同时用没有同源性的植物基因片段为内参照基因 ,制备成“乙、丙型肝炎病毒双检基因芯片”。检测时抽提患者血清中的病毒核酸 ,进行RT- PCR扩增及荧光标记 ,然后与双检基因芯片杂交 ,杂交结果用 Scan Array 30 0 0扫描仪扫描。 结果 :双检基因芯片的内参照结果能做到绝对阳性和绝对阴性 ,同时对不同血清 (正常人血清 ,乙肝 E抗原阳性血清 ,丙肝 RNA阳性血清 ,乙肝、丙肝阳性混合血清 )能有效检测区分。 结论 :本研究成功地制作了乙、丙型肝炎病毒双检基因芯片 。
Objective: To investigate the value of diagnosis microarray in detection of hepatitis B and C virus. Methods: The conservative sequences of hepatitis B and C virus were used as probe. The non homology sequences of vegetative genes were used as control. When it was used, the patient serum was abstracted. The viral nucleotides were reversely transcribed and enlarged by polymerase chain reaction (PCR). Then it was labeled with fluorescence, and hybridized with the diagnosis genechip. The genechips were scanned using scanner (ScanArray 3000). Results: The diagnosis genechip included positive and negative controls. All kinds of serum (normal, E antigen positive, HCV RNA positive, mixed serum of HBV and HCV) could be detected. Conclusion: Diagnosis genechip for HBV and HCV can be used for clinic diagnosis after operators are trained. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第6期527-529,T003,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目 (3 0 0 0 0 0 46)
