骨形态发生蛋白及多孔复合陶瓷人工骨修复长骨大段骨缺损的实验研究

Experimental Study of Using Bone Morphogenetic Protein and Porous Complex Ceramic Artificial Bone to Repair Segment Defect of Long Bone

目的：为研制理想的、能较快修复长骨大段骨缺损的人工骨材料。方法：将骨形态发生蛋白（ＢＭＰ）和多孔复合陶瓷（ＰＣＣ）结合研制成ＢＭＰ／ＰＣＣ人工骨，并将ＢＭＰ／ＰＣＣ和ＰＣＣ人工骨进行兔桡骨大段骨缺损修复的对比研究。术后２、４、８、１２周时取材，分别作大体、组织形态学、新骨形成定量分析及生物力学测试。结果：ＢＭＰ／ＰＣＣ人工骨内新骨形成量明显多于ＰＣＣ人工骨，术后１２周时，ＢＭＰ／ＰＣＣ侧植入部位的抗折强度明显高于ＰＣＣ侧。结论：ＢＭＰ／ＰＣＣ人工骨能更快促进长骨大段骨缺损的修复，是一种较理想的人工骨材料。

To develop ideal artificial bone for accelerating the process of repairing segment defect of long bone. Methods: After bone morphogenetic protein (BMP) was combined with porous complex ceramic (PCC) artificial bone,BMP/PCC complex artificial bone was prepared. BMP/PCC and PCC artificial bone were used in the comparative study of repairing the segment defects of radius diaphysis in rabbits.The samples were harvested in 2,4,8 and 12 weeks, and were then examined by gross, histomorphological analysis, quantitative analysis of new bone formation and biomechanical measurement.Results:The new bone in BMP/PCC artificial bone was more than that in PCC artificial bone. At 12 weeks after operation, the bending strength of the radius in BMP/PCC side was stronger than that in PCC side. Conclusion: BMP/PCC artificial bone could accelerate the repairment of segment defect of long bone. It was an ideal artificial bone substitute.