日本血吸虫(中国大陆株)线粒体相关蛋白对小鼠免疫保护性的初步研究

STUDIES ON IMMUNOPROTECTION IN MICE AFTER IMMUNIZATION WITH SCHISTOSOMA JAPONICUM MITOCHONDRIA-RELATED PROTEIN

目的 研究日本血吸虫 (中国大陆株 )线粒体相关蛋白 (r Sj338/ 2 6 GST)对小鼠的免疫保护性 ,预测其作为血吸虫疫苗候选分子的潜能。方法 将编码日本血吸虫线粒体相关蛋白的 c DNA经PCR扩增后亚克隆入载体质粒 p GEX- 6 p- 1进行表达 ,并用表达的重组抗原的融合蛋白免疫BABL / c小鼠。结果 与对照组相比 ,r Sj338/ 2 6 GST重组蛋白免疫小鼠获得了 32 .3%的减虫率及46 .5 %肝总减卵率 ;与 Sj2 6 GST免疫组相比 ,r Sj338抗原可能使小鼠获得 2 2 .5 %的减虫率及30 .0 %的肝总减卵率。结论 证明重组的日本血吸虫线粒体相关蛋白 (r Sj338/ 2 6 GST及 r Sj338)可诱导宿主抗血吸虫感染的部分保护力 。

Objective To evaluate the immunoprotective effect of Schistosoma japonicum mitochondria related protein( rSj338/26GST). Methods The rSj338/26GST gene fragment was amplified by PCR method and subcloned into expression vector pGEX 6P 1.The expressed recombinant protein was purified and used to immunize the BABL/c mice. Results In BABL/c mice, the recombinant fusion protein of Sj338/26GST could induce 32 3% (P<0 01) worm reduction and 46 5% (P<0 01) total egg reduction. The rSj338 could induce 22 5%(P<0 01) worm reduction and 30 0%(P<0 01) total egg reduction.Conclusion The Sj338/26GST fusion protein are partially effective against S.japonicum, it may be used as a new vaccine candidate.