日本血吸虫单克隆anti-anti-id的建株及初步鉴定

ESTABLISHMENT AND PRELIMINARY IDENTIFICATION OF MONOCLONAL ANTI-ANTI-ID OF SCHISTOSOMA JAPONICUM

目的 制备日本血吸虫抗独特型抗体 (anti- id,Ab2 ) NP30的单克隆抗独特型抗体 (anti-anti- id,Ab3)。方法 应用辣根过氧化物酶标记的 NP30 (HRP- NP30 )免疫 BAL B/ c小鼠的脾细胞与SP2 / 0融合 ,制备单克隆 anti- anti- id。结果 获得 1株稳定分泌抗 NP30的单克隆抗体的细胞株 ,定名为 NP48。NP48的 Ig同型为 Ig G2 b。NP48与 SWAP和 SEA EL ISA反应均阳性。免疫组织化学显示 NP48可定位于虫卵内毛蚴头腺及毛蚴膜。结论 NP48是 NP30的特异性 anti- anti- id,可用于

Objective To establish anti anti idiotypic antibody (anti anti id, Ab 3 ) of monoclonal anti idiotypic antibody (anti id, Ab 2 ) NP30 of Schistosoma japonicum. Methods The anti anti id of NP30 was obtained by fusion of myeloma cells SP2/0 and spleen cells from BALB/c mice immunized by HRP NP30. Results McAb, named NP48, was established. The immunoglobulin isotype of NP48 was determined as IgG 2b . NP48 had positive reaction to SEA and SWAP by ELISA and located at the head gland and membrane of miracidia in eggs of Schistosoma japonicum by the method of immunohistochemistry. Conclusion NP48 is specific anti anti id of NP30 and can be used for isolation and identification of the mimic antigen of NP30.