联合低温保护剂对脐血造血干细胞长期低温保存的作用

The effect of combined cryoprotectant on long term cryopreservation of umbilical blood stem cell

目的 观察联合低温保护剂对脐血造血干细胞长期低温保存的作用。方法 6 0份由联合低温保护剂( 5%DMSO + 6 %HES + 5%人体白蛋白 )保存在岭南脐血库的脐血 ,按时间分为 1 ,2 ,3,4年等 4组 ,复温后观察其细胞活力、总有核细胞数 (TNC)、CD3 4 + 、CD3 4 + CD3 8-、CFU -GM及BFU -E的回收率。结果 6 0例脐血冻存后的细胞活力为96 39% ,TNC ,CD3 4 + ,CD3 4 + CD3 8-,CFU -GM及BFU -E的回收率分别是 95 89% ,94 45% ,88 88% ,87 77%及 83 1 8% ,不同时间组的上述指标之间差异无显著性 (P >0 0 5)。结论 联合低温保护剂具有效果可靠、操作简单等优点 ,适用于脐血造血干细胞的长期低温保存。

Objective To observe the protection of combined cryoprotectant (5%DMSO+6%HES+5%human albumin) on umbilical blood stem cells stored for different cryopreservation duration. Methods 60 cryopreserved umbilical bloods were divided into 4 groups according to duration(1, 2, 3 and 4 years). The viability of cells, total nucleate cells (TNC),CD 34 +, CD 34 +CD 38 -,CFU-GM, and BFU-E were tested before and after cryopreservation with combined cryoprotectant (5%DMSO+6%HES+5%human albumin) . Results The viability of cells,TNC,CD 34 +, CD 34 +CD 38 -,CFU-GM, and BFU-E in 60 cryopreserved umbilical bloods were 96.39%, 95.89%, 94 45%, 88 88%, 87 77% and 83 18% No significant difference were found among different cryopreservation duration groups( P >0 05). Conclusion With the advantages of reliability and simplicity, combined cryoprotectant is desirable long term cryopreservation of umbilical cord blood stem cells