空气或高浓度氧复苏对宫内窘迫胎鼠脑细胞内外钙的影响

Cerebral intra-and extra-cellular calcium concentrations in asphyxiated rat fetuses resuscitated with air or oxygen

目的 比较窒息时空气复苏与高浓度氧复苏的效果 ,为窒息复苏方案的选择提供依据。方法 将 35只胎龄为 2 0d的SD大鼠胎鼠随机分为 3组 ,即假手术组 1 1只 ,制成宫内窘迫模型而给予空气复苏者作为空气复苏组 1 0只 ,给予 92 8%浓度氧复苏者为氧复苏组 1 4只。监测各组胎鼠脑细胞内外钙、钠、钾含量的变化。结果 空气复苏组与高氧复苏组的脑细胞内游离钙离子浓度 [分别为 ( 552 0 8± 93 50 )和 ( 52 0 6 1± 79 0 8)nmol/L]基本一样 ,均明显高于对照组 [( 31 5 2 7± 86 88)nmol/L](P <0 0 0 1 )。各组脑组织总钙、钠、钾含量差异无显著性 (P >0 0 5)。结论 应用空气或92 8%浓度氧进行窒息复苏的效果相似 ,提示临床上可考虑应用空气代替纯氧对窒息新生儿进行复苏。

Objective To compare the effects of resuscitation using room air or oxygen on hypoxic damage of fetal rat brains. Methods Thirty-five fetal rats of twenty-day gestational age were randomly divided into three groups: sham operation (control, n=11 ), room-air resuscitation ( n=10 ), and oxygen (concentration 92.8%)resuscitation ( n=14 ). Fetal rats in the latter two groups suffered from ischemia and hypoxia in-utero resulting from interruption of placental circulation. After recirculation, intra-and extra-cellular concentrations of calcium, sodium, and potassium in the brains were measured in each group. Results Intracellular free calcium concentration of fetal rat brains was similar between the room-air resuscitation group (552.08±93.50 nmol/L) and the oxygen resuscitation group (520.61±79.08 nmol/L), and both were significantly higher than that in the control(315.27±86.88 nmol/L) ( P<0.001 ). There was no difference in the total concentrations of calcium, sodium, or potassium among three groups. Conclusion Resuscitation with room air or 92.8% oxygen have a similar effect on the parameters measured, suggesting that resuscitation of asphyxiated neonates using room air might not be inferior to that using high-concentration oxygen.