大鼠胃伤害性刺激后胃壁一氧化氮合酶的变化

GASTRIC NOXIOUS STIMULATION TO STOMACH INDUCES CHANGES OF THE NUMBER OF NOS POSITIVE NEURONS AND FIBERS IN RATS

目的 :探索胃在受到外源性酸、碱伤害性刺激后 ,胃壁内一氧化氮合酶 (NOS)阳性神经元的数量和染色强度的变化。方法 :分别用 0 6%乙酸和 0 2 %NaOH造成大鼠胃伤害性刺激 ,按不同时程 ,以还原型尼克酰胺腺嘌呤二核苷酸脱氢酶 (NADPH -d)组化法对胃壁NOS阳性神经元的数量和染色强度的变化进行了观察。结果 :胃壁皮区的粘膜下层中都没有观察到NADPH -d阳性神经元 ,胃壁腺区 (特别是泌酸区 )粘膜下层中都观察到阳性神经元。两实验组泌酸区此层内的阳性神经元数约是对照组的 1 4倍 ,P <0 .0 5 ,灌酸组此层内强染色阳性神经元与弱染色阳性神经元的比例由对照组的 1∶1 5变为 1∶2 5 ;灌碱组泌酸区此层内的阳性神经元数强弱比约为 1 5∶1。结论 :胃的外源性酸、碱刺激影响胃粘膜下层中氮能神经元的表达。

NADPH-diaphorase histochemical method was used to investigate the changes of nitric oxide synthase(NOS)in different gastric walls in a rat model of gastric noxious stimulation.The results showed that in the gastric epithelial area of all experimental rats except the gastric glandular area NADPH-d positive neurons were few in the submucosa.In the gastric glandular area,particularly the oxyntic area,a few positive neurons could be found in the submucosa.An increase in the number of NADPH-d positive neurons,being 1-3 h and about 1.4 times higher than the control level 1-3 h following gastric noxious stimulation with 0.6% acetic acid or 0.2% NaOH,was observed in the submucosa of the oxyntic area.The submucous positive neurons displayed a characteristic variation in staining intensity,a three-fifths majority being intensely stained following gastric noxious stimulation with 0.2% NaOH,a five-sevenths majority being weakly stained following gastric noxious stimulation with 0.6% acetic acid.The results demonstrate that gastric noxious stimulation with 0.6% acetic acid or 0.2% NaOH influence the expression of NADPH-d positive neurons in the submucosa of gastric glandular area.The results suggest that NADPH-d positive neurons of the submucosa influence the secretion of gastric gland.