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转基因抗虫油菜的ELISA分析 被引量:5

ELISA Analysis of Transgenic Insect-Resistant Rapeseed
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摘要 为选育高效抗虫油菜新品种 ,采用酶联免疫吸附法检测 Bt杀虫蛋白基因在转基因油菜植株中的表达 .结果表明 ,Bt杀虫蛋白基因在转基因抗虫油菜植株的表达随植株生长时期不同而发生变化 .在第 3~ 9叶期 Bt杀虫蛋白基因稳定、高效地表达 ,Bt杀虫蛋白的表达量为 6 .6 8~ 10 .5 2 ng/ m g(以鲜叶计 ) ,占植物可溶性蛋白的0 .0 6 7%~ 0 .10 5 % .但从第 11叶期后 Bt杀虫蛋白表达量显著地降低 ,只有 0 .2 8~ 2 .0 0 ng/ mg,占植物可溶性蛋白的 0 .0 0 3%~ 0 .0 2 0 % To select and breed a new variety of high yield and high resistance to insects,the expression of Bt insecticidal protein gene in the transgenic rapeseed plants was studied with ELISA.The results show that Bt insecticidal protein gene variedly expresses in the transgenic rapeseed plants with its growth stage.It stably and efficiently expresses during the 3rd~9th leaf stage;its expressing quantity is up to 6.68~10.52 ng/mg fresh leaf;its proportion to plant soluble protein is 0.067%~0.105%. But its expressing quantity apparently decreases after the 11th leaf stage to 0.28~2.00 ng/mg fresh leaf,and its proportion to plant soluble protein is 0.003%~0.020%.
出处 《湖南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2001年第3期179-181,共3页 Journal of Hunan Agricultural University(Natural Sciences)
基金 湖南省科技厅资助项目 (97JKY10 0 5)
关键词 转基因油菜 Bt杀虫蛋白基因 酶联免疫吸附法 ELISA 基因表达 transgenic rapeseed Bt insecticidal protein gene enzyme linked immune sorbent assay
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