副鸡嗜血杆菌人工感染鸡抗体动态的研究

STUDIES ON DYNAMICS OF ANTIBODIES TO HAEMOPHILUS PARAGALLINARUM IN SERA FROM ARTIFICIALLY INFECTED CHICKENS

:本研究采用阻断ELISA(B -ELISA)和血凝抑制试验 (HI)方法 ,检测了鸡体分别人工感染副鸡嗜血杆菌Hp8株 (A型 )和H668株 (C型 )后 0～ 9周的血清抗体消长情况 ,并绘制了示意抗体曲线。结果表明 ,Hp8人工感染后 ,从第 2周到第 9周 ,B -ELISA阳性检出率一直保持 10 0 % ,其中抗体效价在感染后第 4周达到效价高峰 ,平均约为 15 2。而H668人工感染鸡则在感染后第 7周检出率最高 ,此后急剧下降。HI方法对A型抗体的阳性检出率亦在第 4～ 5周达到高峰 ,而对C型抗体的检出率一直较低。结果进一步显示了B -ELISA良好的特异性和敏感性 ,为本方法及其试剂盒产品的进一步应用提供了参考。

The antibodies in sera from chickens being challenged with Haemophilus paragallinarum isolates Hp8 or H668 were measured by B-ELISA and HI,and the antibody curves were also protracted.The results showed that,the positive rate of A-specific antibody has remained 100% from the second week to 9 th week after being challenged with Hp8,the highest titer appeared at 4 th week,the average was about 15.2.But in the chickens challenged with H668,the highest positive rate of C-specific antibody appeared at 7 th week followed by an acute decrease.The highest positive rate of A HI antibody to chickens challenged with Hp8 appeared at 4-5 th week,and the positive rate of C-HI antibody to chickens challenged with H668 was keeping a low level.The results also demonstrated the good specificity and sensitivity of the B-ELISA,and given a reference for the further application of this B-ELISA and correlative products.