含LacZ重组逆转录病毒的制备及其在NIH_3T_3细胞的表达

Construction of Recombinant Retrovirus Encoded LacZ and Its Expression on NIH_3T_3

构建了含有 3.7kbLacZ的重组逆转录病毒载体 ,转染包装细胞系PA317后 ,经G418筛选 ,得到了G418抗性的产毒细胞系 ,用收获的含有重组逆转录病毒粒子的浓缩病毒上清 ,感染NIH3T3细胞 ,经X_gal染色检测 ,发现表达了LacZ的NIH3T3细胞呈蓝色。

The recombinant retrovirus vector pLLSN(carrying LacZ)was constructed and PA317 cells were transfected by the vector.The concentrated viral supernatant from the G418_resistent PA317 was used to infect NIH3T3.The infected NIH3T3 cells were stained by X_gal and those cells which expressed LacZ became blue.