利用酵母提高低聚果糖纯度

Increasing Purity of Fructo oligosaccharides Mixture with Yeast

将黑曲霉AS 0 0 2 3生产的果糖转移酶作用于底物蔗糖 ,可制备含量为 5 5 %的低聚果糖 ,但该产品中亦含有 30 %的副产物葡萄糖 .利用酵母消化低聚果糖产品中的葡萄糖 ,可生产高纯度低聚果糖 .将筛选得到具有较弱转化酶活性的酵母SK 2 .0 0 3,经培养后添加于总糖浓度为 2 0 %的低聚果糖中 ,经 30℃、2 5 0r/min反应 2 4h ,制得纯度为 80 .2 4

A sugar mixture containing 55% fructo oligosaccharides (FOS) could be produced with a fructosyltransferase (FTS) which was extracted from Aspergillus niger AS0023. However, the mixture contains 30% glucose which was an unwanted by product. High purity fructo oligosaccharides can be prepared by consuming glucose with a yeast. A strain of low invertase activity yeast, SK 2.003, was screened out and added to FOS. By incubating at 30 ℃, 250 r/min for 24 h, 80.24% of FOS was obtained.