高效液相色谱法测定接骨胶囊中阿魏酸、补骨脂素及异补骨脂素的含量

Determination of ferulie acid,psoralen and isopsoralen in Jiegu capsule by HPLC

目的 建立以高效液相色谱法测定接骨胶囊中阿魏酸、补骨脂素及异补骨脂素含量的方法。方法 以甲醇 1%冰醋酸(4 0∶6 0 )为流动相 ,于 330nm波长处 ,以外标法测定。结果 阿魏酸在 0 .2 5～ 8.0 μg·mL-1;补骨脂素在 0 .75～ 12 .0 μg·mL-1;异补骨脂素在 1.0～ 16 .0 μg·mL-1范围内 ,浓度与峰面积线性关系良好 (r =0 .9999,0 .9998及 0 .9997)。平均回收率为 :96 .6 0 % ,10 1.1% ,99.6 2 % ;RSD为 0 .86 % ,0 .93% ,0 .74%。结论 此种方法灵敏、准确。

OBJECTIVE: To establish a HPLC method for the determination of feulic acid, psoralen and isopsoralen in Jiegu capsule. METHODS: The samples were extracted with chloroform by ultrasonic agitation and determined by HPLC using methanol-water glacial acetic acid(40:60) as the mobile phase with UV detection at 330 nm. RESULTS: The method was linear over the range of 0.25 ∼ 8.0 μg&middotmL-1(r = 0.9999) for ferulic acid, 0.75 ∼ 12.0 μg&middotmL-1(r = 0.9998) for psoralen and 1.0 ∼ 16.0 μg&middotmL-1 (r = 0.9997) for isopsoralen, respectively. The average recoveries were 96.6 %, 101.1% and 99.62 % with 0.86 %, 0.93 % and 0.74 % of RSD (n = 5), respectively. CONCLUSION: The HPLC method was sensitive, simple and accurate.