紫杉醇提取工艺的放大与优化

Magnification and Optimization of Taxol Isolation Process

紫杉醇的提取工艺包括粉碎、乙醇浸取、氯仿分配、硅胶柱层析、活性碳脱色和结晶等步骤。该文成功地对该工艺进行了放大试验 ,并对各步骤的操作条件进行了优化。加工了 5 t云南红豆杉树皮 ,得纯度 62 .5 %的紫杉醇680 g,收率超过 70 %。

A process of isolating taxol from the bark of Taxus Yunnanensis, which includes extracting the bark with 95% ethanol, extracting the extract with trichloromethane, silica gel column chromatography, decolorizing with activated carbon and recrystallization, was magnified. The operating parameters in the process were optimized. 5000 kg of bark was processed in this work. 680 g crude taxol with a purity of 62.5% was produced. The yield rate was over 70%.