单个体浮游动物脂肪酸的气相色谱分析

GC analysis of fatty acids in individual zooplankton

为使浮游动物脂肪酸测定充分实现规范化及微量化 ,便于浮游动物自然生态及实验生态的研究 ,借助于毛细管气相色谱微量测定技术建立了浮游动物单个体或多个体样品测定的流程及技术 ,并实际测定了采自厦门附近海域的实际浮游动物样品中脂肪酸的含量。虽然这些数据来自几个甚至一个浮游动物样品 ,单个数据仍然显示充分的合理性及可靠性。这些数据显示出浮游动物不同种之间、浮游动物与浮游植物之间都有着明显的不同 ;同时 ,某些种之间或者某些样品之间又存在着各种的联系。这样 ,脂肪酸组成在化学分类学上的作用也得到了明显的体现。

Fatty acids in zooplankton sample, including juvenile crab, Centropages tenuiremis, Artemia naupylii, Pseudeuphysia sinica (and cyrtopia, furcila), Sagitta bedoti, Beroe cucumis, Pleurobrachia globosa, Noctiluca scientillans, collected at Western Xiamen Sea area, were determined. Considering there may be only 1 or less than 1 mg sample for some species, we developed a technological process to make trace analysis of fatty acids (such as C14∶0, C16∶0, C16∶1, C16∶2, C16∶3, C16∶4, C18∶0, C18∶1, C18∶2, C18∶3, C18∶4, C20∶4, C20∶5, C22∶6 et al.). Data of these samples show this method (GC FID) could detect fatty acids of about 0.2μg, and results of two Pseudeuphysia sinica samples are nearly no different. So this process could produce relatively stable result. As chemotaxonomic markers, fatty acids could show the differences between species. Most of zooplankton have C20∶5, C22∶6 and C18∶0 to a certain degree, and C16∶1, C16∶3, C16∶4, C18∶2, C18∶3, C18∶4 do not appear to be main components; some phytoplankton like Dicrateria zhanjiangensis , have more C16∶0, and C18∶3, C18∶1, C16∶4 are their main unsaturated fatty acids. On the other hand, Artemia naupylii also has more C18∶3, C18∶1, C16∶4. With cluster analysis, we found it possible to guess some feeding habit of some zooplankton or even some relation between zooplankton or phytoplankton.