长链DNA在金基底上的固定化和电化学标记

Immobilization of Long-Strand DNA onto Gold Surface and Electrochemical Labeling of Hybridization of DNA

本文提出在金基底上用阳离子聚电解质———聚二烯丙基二甲基胺氯化物 (poly(dial lyldimethylammoniumchloride) ,PDDA)自组装膜固定长链DNA的方法 ,用DiffuseReflectanceIn frared ,XPS和STM技术进行表征 。

Poly(diallyldimethylammonium chloride)(PDDA) was used to facilitate long strand DNA immobilization onto gold surface using self assembly method. Characterization of the PDDA self assembled film and subsequently immobilized DNA were carried out by Diffuse Reflectance Infrared, XPS, STM and electrochemical measurements. Hybridization of DNA was labeled by electrochemical method.