摘要
用硫脲在金电极表面自组装单分子层 ,然后通过戊二醛耦联乙型肝炎表面抗体 (抗 -HBs) ,得到电位型的乙型肝炎抗体膜免疫电极 ,用于检测乙型肝炎表面抗原 (HBs Ag) .叙述了该免疫电极的制备方法、性能特点和临床应用 .对 HBs Ag检测的线性范围是 6～ 3 2 0 μg/L,线性相关系数为 0 .998,检出下限 4μg/L.对40例临床血清检测结果 ,与现行的酶联免疫吸附分析法 (ELISA)的结果比较 ,符合率 87.5 % .
A gold electrode modified by hepatitis B surface antibody covalently bound to self assembled thiourea monolayer has been fabricated to detect the hepatitis B surface antigen. The self assembled monolayer was formed by spontaneous adsorption of thiourea on gold surface and used as a substrate to be modified by anti hepatitis B surface antigen through glutaraldehyde amide reactions. The modification procedure was electrochemically monitored by characterization of the electrode surface. The immunoelectrode and saturated calomel electrode were dipped together into the serum samples diluted by PBS buffer solution (pH=7 4) for the potentiometric determination. More than 87 5 % of the results of the human serum samples obtained by this method were in agreement with those obtained by enzyme linked immunosorbent assay(ELISA). Calibration graphs were linear for HBsAg in range of 6~320 μg/L with correlation coefficient of 0 998 and detection limit of 4 μg/L. The immunoelectrode prepared has good stability and high sensitivity.
出处
《应用化学》
CAS
CSCD
北大核心
2001年第9期705-708,共4页
Chinese Journal of Applied Chemistry
基金
广东省博士启动基金资助课题 ( 99412 8)
