铈诱导悬浮培养的东北红豆杉细胞发生凋亡

Ce^(4+) Induces Apoptosis in Suspension Culture of Taxus Cuspidata Cells

以悬浮培养的东北红豆杉 (Taxuscuspidata)细胞为材料 ,加入一定浓度的Ce4 + ,在不同培养时间取样 ,超薄切片透射电镜观察发现细胞核膜完整、染色质凝聚、趋边化 ;核酸电泳显示DNA发生特异性降解并形成阶梯状电泳条带 (DNAladder) ;应用TUNEL检测方法发现DNA的 3′ OH断端可被特异性标记。Ce4 + 能诱导东北红豆杉细胞发生凋亡。

The standard detection hallmarks of the apoptosis of the Taxus cuspidata cells in suspension culture with Ce 4+ were studied. The condensation and margination of chromatin were observed under the electron microscope. DNA fragmentation ranged 'DNA ladder'on agarose gel electrophoresis. TdT mediated dUTP nick end labeling (TUNEL) analysis of cells reveals that the nuclear DNA strand breaks can be identified by labeling free 3′ OH termini. These results suggest that Ce 4+ induces apoptosis of Taxus cuspidata cells and also indicate that there is a certain relationship between apoptosis and secondary metabolite product Taxol.